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. 2021 Aug 30;12:729560. doi: 10.3389/fpls.2021.729560

FIGURE 2.

FIGURE 2

Blocking miR530 enhances rice blast disease resistance. (A,D) The relative amounts of mature miR530 in the Kasalath control (Ka), the transgenic lines overexpressing MIR530 gene (OX530), or the transgenic lines expressing a target mimic of miR530 (MIM530). The reverse transcription (RT) was carried out with total RNA and miR530-specific stem-loop RT primer (Supplementary Table 1). The RT product was subsequently used as a template for qPCR to detect the amounts of miR530. The amounts of snRNA U6 were detected and used as an internal reference. (B,E) The blast disease phenotypes on leaves of the Kasalath control and the transgenic lines overexpressing miR530 (OX530; B), or the transgenic lines expressing a target mimic of miR530 (MIM530; E) at 5 days postinoculation of the indicated Magnaporthe oryzae strains by punch inoculation, respectively. Bar = 5 mm. (C,F) The relative fungal biomass of indicated strains on the Kasalath control and OX530 (B), or MIM530 (E). The fungal biomass in (C,F) was determined by using the ratio of DNA levels of M. oryzae MoPot2 against that of the rice ubiquitin gene. The data are shown as mean ± SD (n = 3 independent samples). Different letters above the bars indicate significant differences (P < 0.01) as determined by the one-way ANOVA analysis. Similar results were obtained in at least two independent experiments.