FIGURE 3.

miR530 regulates Magnaporthe oryzae-induced defense responses. (A) The relative mRNA levels of the defense-related genes (OsNAC4, OsPR1A, and OsPR10b) in the Kasalath controls (Ka) and the indicated lines at the indicated time points. The data are shown as mean ± SD (n = 3 independent samples). The mRNA levels were normalized to that in the indicated control. Different letters above the bars indicate significant differences (P < 0.01) as determined by the one-way ANOVA analysis. (B) The hydrogen peroxide (H₂O₂) accumulation in the Kasalath controls and indicated lines at 48 h postinoculation of M. oryzae strain Guy11. The accumulation of H₂O₂ was stained by 3,3′-diaminobenzidine (DAB), and the intensity of brown indicates the amounts of H₂O₂. The red arrows indicate appressoria formed from conidia. The photographs given above were taken with the leaves using a microscope (Zeiss imager A2, Germany). Scale bars = 10 μm. The photographs given below were taken with the leaves with a stereomicroscope (Zeiss imager A2, Germany). Scale bars = 50 μm. These experiments were repeated two times with similar results.