FIGURE 2.
LncRNA GAS5 regulated HG-induced EMT through miR-21/PTEN. (A) Western blotting showed that PTEN was downregulated in HG-induced EMT. (B) Compared with HG group, overexpression of lncRNA GAS5 upregulated PTEN expression. (C) Compared with HG group, lncRNA GAS5 siRNA downregulated PTEN expression. (D) Real-time PCR showed that miR-21 was upregulated in HG-induced EMT, compared with HG group, overexpression of lncRNA GAS5 downregulated miR-21 and lncRNA GAS5 siRNA upregulated miR-21. Each value represents the mean ± SEM (n = 3) (∗∗P < 0.01 vs. control, ##P < 0.01 vs. HG).