Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Aug 30;12:654951. doi: 10.3389/fphys.2021.654951

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 Fan, Zhao, Ma and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

The Wnt/β-catenin pathway was stimulated in lncRNA GAS5/miR-21/PTEN-mediated EMT. (A) HG stimulated the Wnt/β-catenin pathway. (B) lncRNA GAS5 overexpression significantly downregulated the Wnt/β-catenin pathway. (C) lncRNA GAS5 siRNA significantly upregulated the Wnt/β-catenin pathway. (D) Immunofluorescence staining demonstrated that an increase of nuclear β-catenin accumulation was evident in HG-induced HPMCs EMT, lncRNA GAS5 overexpression attenuated β-catenin nuclear localization and lncRNA GAS5 siRNA aggravated nuclear localization. The arrows indicate nuclear localization of β-catenin. (E) miR-21 inhibitor attenuated lncRNA GAS5 siRNA stimulated Wnt/β-catenin pathway. (F) PTEN reversed lncRNA GAS5 siRNA stimulated Wnt/β-catenin pathway. Each value represents the mean ± SEM (n = 3) (**P < 0.01 vs. control, ^##P < 0.01 vs. HG, ^&⁣&P < 0.01 vs. siGAS5).