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. 2021 Aug 30;13:685594. doi: 10.3389/fnagi.2021.685594

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Copyright © 2021 Griffiths, Abdel-Khalik, Moore, Wijeyekoon, Crick, Yutuc, Farrell, Breen, Williams-Gray, Theofilopoulos, Arenas, Trupp, Barker and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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LC-MS(MSⁿ) analysis of dihydroxycholesterols, dihydroxycholestenones, trihydroxycholestenones, and hydroxybisoxocholestenoic acid in CSF. (A) RIC of 555.4317 ± 5 ppm (upper panel) and 550.4003 ± 5 ppm (lower panel) revealing 7α,25-diHC, 7α,26-diHC, 7α,25-diHCO, and 7α,26-diHCO. MS³ (M⁺ → M⁺-Py→) spectra revealing (B) 7α,25-diHC and (C) 7α,26-diHC. (D) RIC of 566.3952 ± 3 ppm corresponding to 7α,x,y-triHCO. (E) High resolution (100,000) mass spectrum (upper panel) and theoretical mass spectrum (lower panel) of 7α,x,y-triHCO. (F) RIC of 578.3588 ± 3 ppm corresponding to 7αH-3,24-diO-CA. (G) High resolution (100,000) mass spectrum (upper panel) and theoretical mass spectrum (lower panel) of 7αH,3,24-diO-CA.