. 2021 Aug 30;13:685594. doi: 10.3389/fnagi.2021.685594

TABLE 2.

Oxysterols in CSF of PD patients and controls.

Fraction A	Fraction B	Sterol systematic name (common name)	Abbreviation	Study 1					Study 2					Note
m/z	m/z			ng/mL					ng/mL

				PD		Control		Significance	PD		Control		Significance

				Mean	SD	Mean	SD	PD vs Control	Mean	SD	Mean	SD	PD vs Control
527.3640	522.3326	7α-Hydroxy-3-oxochol-4-en-24-oic acid	7αH,3O-Δ⁴-BA	0.672	0.246	0.708	0.237	NS	0.675	0.167	0.551	0.081	NS
539.4004	534.369	7α-Hydroxy-27-nor-cholest-4-ene-3,24-dione	7αH-27-nor-C-3,24-diO	0.387	0.162	0.245	0.143	**	0.698	0.194	0.638	0.025	NS	1
539.4368	NA	Cholest-5-ene-3β,24S-diol (24S-hydroxycholesterol)	24S-HC	0.050	0.022	0.045	0.019	NS	0.015	0.009	0.008	0.004	NS
539.4368	NA	Cholest-5-ene-3β,25-diol (25-hydroxycholesterol)	25-HC	0.028	0.028	0.030	0.019	NS	0.016	0.015	0.012	0.005	NS
539.4368	NA	Cholest-5-ene-3β,(25R)26-diol ((25R),26-Hydroxycholesterol)	26-HC	0.113	0.064	0.100	0.028	NS	0.093	0.053	0.064	0.017	NS
539.4368	NA	Cholest-5-ene-3β,7β-diol (7β-Hydroxycholesterol)	7β-HC	0.056	0.066	0.036	0.027	NS	0.181	0.474	0.082	0.034	NS	2
539.4368	534.4054	3β-Hydroxycholest-5-en-7-one (7-Oxocholesterol)	7-OC	0.601	0.513	0.378	0.225	NS	0.671	0.671	0.731	0.540	NS	2
539.4368	NA	Cholest-5-ene-3β,7α-diol (7α-Hydroxycholesterol)	7α-HC	0.063	0.067	0.039	0.032	NS	0.091	0.118	0.056	0.027	NS	2
539.4368	NA	Cholest-5-ene-3β,6β-diol (6β-Hydroxycholesterol)	6β-HC	0.345	0.234	0.280	0.312	NS	0.918	1.289	0.593	0.141	NS	3
537.4212	NA	9,10-Secocholesta-5,7,10-triene-3β,25-diol (25-hydroxyvitamin D₃)	25-D₃	NM	NM	NM	NM	NA	0.171	0.095	0.140	0.057	NS
551.4004	546.369	3-Oxocholesta-4,6-dien-26-oic acid	–	2.654	2.426	1.546	0.468	NS	1.461	0.496	1.154	0.335	NS	4
551.4004	NA	3β-Hydroxycholesta-5,7-dien-26-oic acid	–	0.318	0.334	0.079	0.099	**	0.142	0.143	0.043	0.038	NS	5
553.4161	NA	3β,x-Dihydroxycholest-5-en-y-one	3β,x-diHC-yO	NM	NM	NM	NM	NA	0.050	0.036	0.066	0.028	NS	6
553.4161	NA	3β-Hydroxycholest-5-en-(25R)26-oic acid	3β-HCA	1.073	0.793	0.959	0.416	NS	1.210	0.557	0.899	0.287	NS
555.4317	550.4003	7α,25-Dihydroxycholest-4-en-3-one	7α,25-diHCO	NM	NM	NM	NM	NA	0.009	0.005	0.006	0.001	*
555.4317	NA	Cholest-5-ene-3β,7α,25-triol (7α,25-Dihydroxycholesterol)	7α,25-diHC	NM	NM	NM	NM	NA	0.006	0.005	0.006	0.004	NS
555.4317	550.4003	7α,(25R)26-Dihydroxycholest-4-en-3-one	7α,26-diHCO	NM	NM	NM	NM	NA	0.009	0.004	0.005	0.001	**
555.4317	NA	Cholest-5-ene-3β,7α,(25R)26-triol (7α,(25R)26-Dihydroxycholesterol)	7α,26-diHC	NM	NM	NM	NM	NA	0.005	0.002	0.002	0.002	*
567.3953	562.3639	x-Hydroxy-3-oxocholesta-4,6-dien-26-oic acid	–	0.190	0.169	0.112	0.041	NS	0.453	0.143	0.361	0.119	NS
567.3953	562.3639	x-Hydroxy-3-oxocholesta-4,6-dien-26-oic acid	–	0.100	0.090	0.069	0.036	NS	NM	NM	NM	NM	NA
569.4110	NA	3β,7β-Dihydroxycholest-5-en-26-oic acid	3β,7β-diHCA	0.455	0.212	0.403	0.190	NS	0.506	0.169	0.406	0.104	NS	7
569.4110	NA	3β,x,y-Trihydroxycholest-5-en-z-one	3β,x,y-triHC-zO	0.228	0.122	0.147	0.067	*	0.172	0.061	0.127	0.036	NS	8
569.4110	564.3796	7α-Hydroxy-3-oxocholest-4-en-26-oic acid	7αH,3O-CA	22.728	11.445	15.851	4.305	*	21.198	6.292	17.731	3.983	NS	7,9
569.4110	NA	3β,7α-Dihydroxycholest-5-en-26-oic acid	3β,7α-diHCA	3.235	3.308	2.042	1.577	NS	3.808	2.258	1.785	1.575	NS	7,10
571.4266	566.3952	7α,x,y-Trihydroxycholest-4-en-3-one	7α,x,y-triHCO	0.198	0.258	0.286	0.116	NS	0.116	0.062	0.068	0.013	*	11
583.3903	578.3589	7α-Hydroxy-3,24-bisoxocholest-4-en-26-oic acid	7αH,3,24-diO-CA	0.236	0.082	0.208	0.057	NS	0.285	0.094	0.227	0.065	NS	12
585.4059	580.3745	7α,24-Dihydroxy-3-oxocholest-4-en-26-oic acid	7α,24-diH,3O-CA	NM	NM	NM	NM	NA	0.312	0.080	0.251	0.021	NS
585.4059	580.3745	7α,x-Dihydroxy-3-oxocholest-4-en-26-oic acid	7α,x-diH,3O-CA	5.212	1.737	2.938	0.887	***	5.938	1.522	5.038	1.314	NS	13
585.4059	580.3745	7α,25-Dihydroxy-3-oxocholest-4-en-26-oic acid	7α,25-diH,3O-CA	1.306	0.472	0.715	0.224	***	1.634	0.458	1.353	0.213	NS
585.4059	580.3745	7α,12α-Dihydroxy-3-oxocholest-4-en-26-oic acid	7α,12α-diH,3O-CA	NM	NM	NM	NM	NA	1.100	1.176	1.157	0.600	NS
601.4008	596.3694	Trihydroxy-3-oxocholest-4-en-26-oic acid	triH,3O-CA	0.021	0.063	0.077	0.041	*	NM	NM	NM	NM	NA
		TOTAL 7α-Hydroxy-3-oxocholest-4-en-26-oic acid	7αH,3O-CA	25.383	13.206	17.396	4.628	*	22.659	6.745	18.886	4.312	NS	14
		TOTAL 3β,7α-Dihydroxycholest-5-en-26-oic acid	3β,7α-diHCA	3.553	3.477	2.121	1.648	NS	3.950	2.384	1.828	1.606	NS	15

*P < 0.05, **P < 0.01, ***P < 0.001 determined using Mann–Whitney Test. NA, not applicable. As a visual aid concentrations are written in bold. 1. Decarboxylation product of 7α-hydroxy-3,24-bisoxocholest-4-en-26-oic acid. 2. May be formed enzymatically or by in vivo or ex vivo autoxidation. 3. 6β-HC is the dehydration product of cholestane-3β,5α,6β-triol, formed from 5,6-epoxycholesterol. 4. Dehydration product of 7αH,3O-CA. 5. Dehydration product of 3β,7-diHCA. 6. x and y probably correspond to 22 and 24 or 20 and 22, based on MS³ spectra. 7. 25R and 25S epimers measured in combination. 8. x, y and z probably 22, 25 and 24. 9. Some dehydration of 7αH,3O-CA (see 4). 10. Some dehydration of 3β,7-diHCA (see 5). 11. x any y probably 24,25, 24,26, or 25,26. 12. Undergoes decarboxylation to 7α-hydroxy-27-nor-cholest-4-ene-3,24-dione (see 1). 13. x is probably on the side-chain. 14. Total 7α-hydroxy-3-oxocholest-4-en-26-oic acid is a combination of molecule and its dehydrated analogue. 15. Total 3β,7α-dihydroxycholest-5-en-26-oic acid is a combination of molecule and its dehydrated analogue.