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. 2021 Aug 17;18(15):3506–3515. doi: 10.7150/ijms.63564

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Knockdown of circ_0000673 promoted endometrial cell proliferation and migration. (A) Identification of endometrial stromal cells. Endometrial stromal cells presented a fusiform cell morphology. Vimentin-positive expression by immunofluorescence staining confirmed the presence of endometrial stromal cells. (B) The fluorescence of green fluorescent protein was used to evaluate transfection efficiency and showed that the cells were successfully transfected by circ_0000673 lentiviral vectors (green). Blue represents the nuclei. (C) qPCR confirmed significant downregulation of circ_0000673 after transfection with LV-circ_0000673-1/2 in endometrial stroma cells. (D) The CCK-8 experiment showed that the cell proliferation abilities of endometrial stromal cells were significantly increased after circ_0000673 knockdown. (E) Clone formation assays demonstrated that cell vitality in the group transfected with circ_0000673 lentiviral vectors was higher than that in the control group. (F) Wound healing assays showed that the cell migration capacity was enhanced by circ_0000673 knockdown. * Mean compared with negative control group, P < 0.05.

Knockdown of circ_0000673 promoted endometrial cell proliferation and migration. (A) Identification of endometrial stromal cells. Endometrial stromal cells presented a fusiform cell morphology. Vimentin-positive expression by immunofluorescence staining confirmed the presence of endometrial stromal cells. (B) The fluorescence of green fluorescent protein was used to evaluate transfection efficiency and showed that the cells were successfully transfected by circ_0000673 lentiviral vectors (green). Blue represents the nuclei. (C) qPCR confirmed significant downregulation of circ_0000673 after transfection with LV-circ_0000673-1/2 in endometrial stroma cells. (D) The CCK-8 experiment showed that the cell proliferation abilities of endometrial stromal cells were significantly increased after circ_0000673 knockdown. (E) Clone formation assays demonstrated that cell vitality in the group transfected with circ_0000673 lentiviral vectors was higher than that in the control group. (F) Wound healing assays showed that the cell migration capacity was enhanced by circ_0000673 knockdown. * Mean compared with negative control group, P < 0.05.