FIG. 3.

Relative binding of the PTB domain to 17-residue peptides based on the APP sequence. (A) Substitution of alanine for each amino acid in turn. The original amino acid is indicated below the histogram. (B) Retesting of each alanine substitution that resulted in reduced binding with 19 amino acids. The positions of the substitutions are indicated in each window by underlining of the wild-type residue. Substituted amino acids are listed below. Phosphotyrosine (pY) was substituted at positions −5 and 0.