BI2536-treated tumor cells were phagocytosed and could promote BMDC activation. A. Schematic diagram of co-culture system illustrating the effect of BI2536-treated tumor cells on BMDC function. B. Representative immunofluorescence images showing nucleus (blue, DAPI), BMDCs (red, DiI) and BI2536-treated LLC cells (green, CFSE) 0, 1 and 2 h after co-culture (confocal imaging, objective: 20×). The arrow indicates the surrounding BMDCs ready to engulf tumor cells. Scale bars, 20 µm. C. The phagocytosis of CD11c+ DCs after co-culture with BI2536-treated LLC cells (CFSE), as assessed by flow cytometry. D-F. The expression of CD80, CD86 and MHCII in BMDC after incubation with treated tumor cells. G-H. LLC cells treated with BI2536 were inoculated subcutaneously into mice and the activation of DCs in inguinal lymph nodes was flow cytometrically detected at different time points after vaccination. The MFI of CD80 and CD86 on CD11c+ cells are listed.