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. 2021 Sep 2;24(5):758. doi: 10.3892/mmr.2021.12398

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Copyright: © Yuan et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Relationship between TGF-β2 and miR-29a. (A) The binding site between miR-29a and the 3′UTR of TGF-β2 mRNA was predicted by the StarBase dataset. (B) Dual-luciferase reporter assay results indicated that miR-29a can directly bind to the TGF-β2 3′UTR. Two-way ANOVA followed by Bonferroni post hoc test was used to compare the differences among groups. ***P<0.001 vs. mimics-NC group. (C) Effect of miR-29a overexpression and knockdown on the expression level of TGF-β2 in HSFBs. One-way ANOVA followed by Bonferroni post hoc test was used to compare the differences among different groups. *P<0.05, **P<0.01 vs. NC group; ^###P<0.001 vs. miR-29a mimics group. miR, microRNA; wt, wild-type; mut, mutant; UTR, untranslated region; NC, negative control; ns, not significant.