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. 2021 Sep 7;24(5):778. doi: 10.3892/mmr.2021.12418

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Copyright: © Pan et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — LBP counteracts I/R-induced decrease of viability, promotion of apoptosis, cardiomyocyte damage, inflammation and oxidative stress in H9C2 cells. (A). The viability of I/R-induced H9C2 cells treated with LBP (15, 30 and 60 µg/ml) was determined using an MTT assay. (B) The apoptosis of I/R-induced H9C2 cells treated with LBP (15, 30 and 60 µg/ml) was determined via (C) flow cytometry. The levels of (D) LDH, (E) CK, (F) IL-1β, (G) IL-6, (H) TNF-α, (I) MDA and (J) SOD in I/R-induced H9C2 cells treated with LBP (15, 30 and 60 µg/ml) were measured using ELISAs. ***P<0.001 vs. Blank; ^{^}P<0.05, ^{^^}P<0.01, ^{^^^}P<0.001 vs. I/R. I/R, ischemia/reperfusion; LBP, Lycium barbarum polysaccharide; LDH, lactate dehydrogenase; CK, creatine kinase; MDA, malondialdehyde; SOD, superoxidase dismutase.