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. 2021 Sep 7;24(5):778. doi: 10.3892/mmr.2021.12418

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Copyright: © Pan et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 6. — LBP reverses RAPA-induced inhibition on Nrf2 nuclear expression in I/R-induced H9C2 cells. The nuclear (A) mRNA and (B) protein expression levels of Nrf2 in I/R-induced H9C2 cells with LBP (15 µg/ml) and RAPA treatment in combination or alone were calculated via RT-qPCR and western blotting. Lamin A was the internal control. The cytosolic (C) mRNA and (D) protein expression levels of Nrf2 in I/R-induced H9C2 cells treated with LBP (15 µg/ml) and RAPA in combination or alone were measured via RT-qPCR and western blotting, with GAPDH serving as an endogenous reference. **P<0.01, ***P<0.001 vs. I/R; ^{^^^}P<0.001 vs. LBP-15; ^###P<0.001 vs. RAPA. Nrf2, nuclear factor-erythroid factor 2-related factor 2; I/R, ischemia/reperfusion; LBP, Lycium barbarum polysaccharide; RAPA, rapamycin; RT-qPCR, reverse transcription-quantitative PCR.