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. 2021 Sep 3;24(5):769. doi: 10.3892/mmr.2021.12409

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Copyright: © Liang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — LBP protects cells from H₂O₂-induced apoptosis. (A-E) Apoptosis was detected via staining with Annexin V-FITC and PI. Flow cytometric analysis of ARPE-19 cells in each group. (F) Quantification of the extent of apoptosis in each group. (G) Western blot analysis was performed to detect the levels of apoptosis-related proteins (cleaved caspase-3, Bax and Bcl-2). Data are presented as the mean ± SEM (n=3). **P<0.01, ***P<0.001 vs. control; ^#P<0.01, ^##P<0.001 vs. H₂O₂-treated cells with no LBP pretreatment. LBP, Lycium barbarum polysaccharide; PI, propidium iodide.