Table 3. In Vitro Potency of Key Analogues in the B. malayi mf Assay.
| molecule | anti-Wolbachia potency from cell assay EC50 (nM) | anti-Wolbachia potency from mf assay (6 days at 5 μM % Wolbachia reduction in wsp:gsta) |
|---|---|---|
| DOX | 17 | 86.5 |
| 1 | 21 | 75.60 |
| 3 | 17 | 83.20 |
| 10d | 105 | 77.10 |
| 15f | 143 | 80.40 |
| 15i | 51 | toxicb |
| 15j | 52 | 85 |
a
wsp, Wolbachia surface protein copy number, median % reduction cf. vehicle (DMSO) control; gst, GST copy number (single copy gene, worm size biomarker).
b
Significantly reduced motility of the mf after 6 days incubation comparing with vehicle (DMSO) control.