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Disruption of PCNA-cdk4 complexes in E1A-expressing C2 cells. Cell lysates prepared from C2 or from E1A-expressing C2 cells cultured in differentiation medium for 48 h were immunoprecipitated with the anti-cdk4 antibody. Immunoprecipitated proteins were then resolved by SDS-PAGE and analyzed by immunoblotting by using antibodies specific for p21, cyclin D3, or PCNA. The levels of PCNA in each cell line were detected by immunoblot analysis of whole-cell lysates (WCL).
