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Schematic of the Microbubbling SARS-CoV-2 Antigen Assay. NP swab eluant is first treated with lysis buffer to release N proteins from SARS-CoV-2 viruses. N protein is then detected by the smartphone-based microbubbling digital assay. The microbubble images are quantified and classified as positive or negative by computer vision and ML algorithms. Scale bar, 500 μm.
