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. 2021 Sep 13;40:287. doi: 10.1186/s13046-021-02081-8

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — SLC2A1-AS1 competitively absorbs miR-378a-3p by ceRNA mechanism in ESCC cells. A. qRT-PR assay of subcellular SLC2A1-AS1 level in the nucleus and cytoplasm of EC9706, TE1 and KYSE180 cells, GAPDH and U6 are employed as endogenous controls. B. Subcellular localization of SLC2A1-AS1 in EC9706, TE1 and KYSE180 cells is investigated by FISH experiment, SLC2A1-AS1 is labeled by Cy3 (red) and nuclei are stained with DAPI (blue). C. The miR-378a-3p binding sites in SLC2A1-AS1 transcript are predicted by DIANA LncBase Predicted v.2. D. The correlation of SLC2A1-AS1 with miR-378a-3p is examined in ESCA samples using the bioinformatics tool StarBase E. Double luciferase report experiment is performed to verify the interaction of SLC2A1-AS1 with miR-378a-3p by co-transfecting pmirGLO-SLC2A1-AS1-WT or pmirGLO-SLC2A1-AS1-MUT along with NC-mimic or miR-378a-3p mimic into ESCC cells, compared to NC group, **P < 0.01 and ****P < 0.0001, indicating statistical significance. F. Fold enrichment of SLC2A1-AS1 by Ago2 antibody or IgG in EC9706, TE1 and KYSE180 cells. G. Fold enrichment of miR-378a-3p by Ago2 antibody or IgG in EC9706, TE1 and KYSE180 cells. H. Enrichment of SLC2A1-AS1 in EC9706, TE1 and KYSE180 cells transfected with NC mimic or miR-378a-3p mimic, (F, G and H, compared to IgG group, ****P < 0.0001, indicating statistical significance). I. SLC2A1-AS1 depletion promotes the expression of miR-378a-3p in EC9706, TE1 and KYSE180 cells compared to NC group, ****P < 0.0001, indicating statistical significance. J. SLC2A1-AS1 overexpression suppresses the expression of miR-378a-3p in EC9706, TE1 and KYSE180 cells, compared to pcDNA3.1, ****P < 0.0001, indicating statistical significance