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. 2021 Sep 13;40:287. doi: 10.1186/s13046-021-02081-8

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — The expressions of miR-378a-3p and Glut1, and Glut1 is a direct target of miR-378a-3p in ESCC cells. A. The expression of miR-378a-3p is detected in 162 cases of ESCA samples and 11 cases of normal samples by StarBase online software. B. The miR-378a-3p level is investigated in 119 cases of ESCC tissues and paired normal tissues by GEO dataset GSE43732. C. The miR-378a-3p level is determined in 60 cases of ESCC tissues and paired normal tissues by qRT-PCR, compared to normal tissues, ****P < 0.0001, indicating statistical significance. D. The expression of miR-378a-3p is examined by qRT-PCR in a panel of ESCC cells, compared to Het-1A cell, **P < 0.01, ***P < 0.001 and ****P < 0.0001, indicating statistical significance. E. The expression of Glut1 is detected in 162 cases of ESCA samples and 11 cases of normal samples by StarBase online software. F. The Glut1 level is investigated in 7 cases of ESCC tissues and paired normal tissues by GEO Dataset GSE111011. G. Western blot assay for Glut1 protein expression in a panel of ESCC cells and normal esophageal epithelial cell Het-1A. H. Quantification of relative levels of Glut1 in a number of ESCC cells and normal esophageal epithelial cell Het-1A, compared to Het-1A cell, *P < 0.05, **P < 0.01 and ****P < 0.0001, indicating statistical significance. I. IHC assay for Glut1 protein expression in 60 cases of ESCC tissues and paired normal tissues. J. The miR-378a-3p binding sites in Glut1 transcript are predicted by miRDB online software. K. Double luciferase report experiment is carried out to confirm the interaction of Glut1 with miR-378a-3p by co-transfecting pmirGLO-Glut1-WT or pmirGLO-Glut1-MUT as well as NC-mimic or miR-378a-3p mimic into ESCC cells. L. miR-378a-3p mimic upregulates the expression of miR-378a-3p, whereas miR-378a-3p inhibitor downregulates the miR-378a-3p level in ESCC cells. M. miR-378a-3p mimic suppresses the expression of Glut1 protein in ESCC cells. N. The analysis of relative level of Glut1 protein after transfection with NC mimic or miR-378a-3p mimic in ESCC cells. O. miR-378a-3p inhibitor enhances the expression of Glut1 protein in ESCC cells. P. The assay of relative level of Glut1 protein after transfection with NC inhibitor or miR-378a-3p inhibitor in ESCC cells. (K, L and N, compared to NC-mimic or NC-inhibitor, **P < 0.01, ***P < 0.001 and ****P < 0.0001, indicating statistical significance)