Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Sep 11;21:1017. doi: 10.1186/s12885-021-08751-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 3 — Estrogen reduces circulating low-density neutrophils (LDN) in tumour-bearing mice. A, Schematic diagram of the discontinuous Percoll gradient used for the separation of blood into the different cell fractions. B-E, Experiment is conducted according to the description in Fig. 1. B, Percentage of circulating LDN (CD45⁺CD11b⁺Ly6G⁺) out of viable CD45⁺ population via flow cytometry analysis, Null+Ctrl (N.T) n = 3, Null+Ctrl n = 8, Null+E2V n = 8, Inv + Ctrl (N.T) n = 6, Inv + Ctrl n = 12, Inv + E2V n = 13. C, Estrogen treatment significantly reduces the amount of circulating LDN in tumour-bearing mice while not significantly affecting circulating HDN; (i) Calculated number of circulating LDN per ml blood, (ii) Calculated number of circulating HDN per ml blood. Null+Ctrl (N.T) n = 3, Null+Ctrl n = 5, Null+E2V n = 5, Inv + Ctrl n = 5, Inv + E2V n = 6. D, Representative image of Giemsa stained mature (multilobed nucleus) and immature (banded nucleus) neutrophils. Scale bar: 50um. E, Estrogen treatment in tumour-bearing mice does not affect the proportion of mature and immature neutrophils in circulating LDN; (i) Distribution of circulating LDN displaying banded or multilobed nucleus, (ii) Distribution of circulating HDN displaying banded or multilobed nucleus. Inv + Ctrl n = 7, Inv + E2V n = 7, Null+Ctrl n = 3, Null+E2V n = 3. F-H, At Inv D2, OVX mice were inoculated with 4 T1-Luc2 cells at the 9th abdominal mammary gland. At 48 h prior to sample collection, mice were treated with either E2V or Ctrl. Mice were euthanized and tumour harvested at day 14 post-tumour inoculation. F, Estrogen treatment for 48 h in tumour-bearing Inv mice led to an increased proportion of total circulating neutrophils; Data are expressed as percentage of circulating neutrophils (CD45⁺CD11b⁺Ly6G⁺) out of viable CD45⁺ population via flow cytometry analysis, Inv + Ctrl n = 3, Inv + E2V n = 4. G, Estrogen treatment for 48 h in tumour-bearing Inv mice resulted in a small reduction in circulating LDN while HDN remain unchanged; Data are expressed as percentage of circulating LDN (CD45⁺CD11b⁺Ly6G⁺) out of viable CD45⁺ population via flow cytometry analysis, Inv + Ctrl n = 3, Inv + E2V n = 4. H, (i) Number of circulating LDN per ml blood, (ii) Number of circulating HDN per ml blood. Data represented as mean ± SEM. Statistical significances determined using one-way ANOVA followed by post-hoc tukey test in (B) and (C); two-tailed unpaired student’s T-test in (F-H)