Fig. 5.

Extended estrogen treatment increases pro-tumoral markers expression in both circulating LDN and HDN of tumour-bearing mice. a Experiment is conducted according to the description in Fig. 3F-H. Treatment with estrogen for 48 h did not led to an increase in pro-tumoral markers in tumour-bearing Inv mice; Gene expression of pro-tumoral markers Arg1, Il1b, Ccl5 and Tgfb1 relative to Gapdh in isolated LDN and HDN by qPCR, Inv + Ctrl n = 3, Inv + E2V n = 3. b Experiment is conducted according to the description in Fig. 1. Gene expression of pro-tumoral markers Arg1, Il1b, Ccl5, and Tgfb1 relative to Gapdh in isolated LDN and HDN by qPCR, Null+Ctrl n = 8, Null+E2V n = 8, Inv + Ctrl n = 11, Inv + E2V n = 12, Inv + Ctrl (N.T) n = 8, Inv + E2B (N.T) n = 4. Data represented as mean ± SEM. Statistical significances determined using two-tailed unpaired student’s T-test