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. 2021 Sep 13;28:63. doi: 10.1186/s12929-021-00759-x

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Characterization of the tumor microenvironment in spontaneous HPV16-E6E7-expressing oral tumor model in HLA-A2 (AAD) transgenic mice treated with CRT/E7(N53S) DNA vaccine. HPV16-E6E7-expressing oral tumors were induced in HLA-A2 (AAD) mice (4 per group) and treated as described in Fig. 5. One week following the final vaccination, the tumor was surgically extracted for analysis. A Determination of E7(aa11-20) peptide-specific CD8 + T cells using E7 (aa11-20) peptide loaded HLA-A2 tetramer staining followed by flow cytometry analysis. Upper panel is an example of flow cytometry. Bottom panel is vertical scatter plot summarizing the number of E7(aa11-20) peptide-specific CD8 + T cells in the tumor of mice vaccinated with CRT/E7(N53S) or empty vector. B Determination of CD8 + T TILs by flow cytometry analysis. Upper panel is an example of flow cytometry. Bottom panel is the vertical scatter plot summarizing the percent of CD8 + TILs. C Determination of CD4 + TILs followed by flow cytometry analysis. Upper panel is an example of flow cytometry. Bottom panel is the vertical scatter plot summarizing the percent of CD4 + TILs. D Determination of MDSCs by flow cytometry analysis. Upper panel is example of flow cytometry. Bottom panel is the vertical scatter plot summarizing the percent of MDSCs