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. 2021 Mar 23;13(6):433–444. doi: 10.1093/jmcb/mjab018

Figure 3.

Figure 3

TRC express higher levels of MCAD and CPT-1A regulated by AMPK and ERRα/PGC-1β. (A) The cells were treated with vehicle or endoxifen (5 µM) for 24 h. Lysates were immunoblotted for the indicated proteins. (B‒E) The cells were transfected with control siRNA, ERRα siRNA (pool, B and C), or PGC-1β siRNA (pool, D and E) and then treated with vehicle or endoxifen (5 µM) for 24 h. (B and D) mRNA expression was analyzed for the indicated genes. There are significant differences (P < 0.05) in MCAD and CPT-1A mRNA levels between endoxifen-treated MCF7 cells and TRC (B), in MCAD but not CPT-1A mRNA levels between control siRNA and ERRα siRNA-transfected MCF7 cells and TRC (B), and in MCAD and CPT-1A mRNA levels between control siRNA and PGC-1β siRNA-transfected MCF7 cells and TRC treated with endoxifen (D). (C and E) Lysates were immunoblotted for the indicated proteins. Relative density of the proteins normalized to loading control is indicated below the blots. (F) The cells were transfected with control siRNA, individual pairs of ERRα siRNA (#1 and #2), or individual pairs of PGC-1β siRNA (#1 and #2) and then treated with vehicle or endoxifen (5 µM) for 24 h. Lysates were immunoblotted for the indicated proteins.