FIGURE 3.

Stimulation of glycine conjugation regulates hepatic glycine metabolism. A‐G, Accumulation of glycine and derivatives in liver of Gldc‐deficient mice is partially corrected by benzoate administration. A, Heatmap and fold‐changes for glycine and glycine derivatives in liver of adult wild‐type and Gldc ^GT1/GT1 mice with/without administration of benzoate (n = 6 per group). B‐G, Mass spectrometry data for glycine and selected derivatives. H, Clustering on the basis of abundance of glycine derivatives in liver separates benzoate‐treated Gldc ^GT1/GT1 from untreated Gldc ^GT1/GT1. I‐J, Relationship between glycine abundance (scaled) and I, guanidinoacetate or J, isovalerylglycine. K, Benzoate treatment leads to significant accumulation of hippurate in liver (**significantly different from wild‐type, P < .05; *significantly different from untreated Gldc ^GT1/GT1, P < .05 ANOVA)