FIG. 2.

Relative rates of translation in various translation initiation mutants. The rate of [³⁵S]methionine incorporation was measured either at 24°C or after a 1-h temperature shift to 38°C. The rates of translation are given relative to the wild-type strain at 24°C (where rates were originally measured as the rate of incorporation per optical density unit after 30 min). The rate of translation for each strain is the average of at least two independent experiments. The numbers in parentheses are the rates of incorporation of the strains at 38°C relative to the wild-type strain at 38°C. ts, temperature sensitive.