FIG. 3.

mRNAs from strains containing a mutation in eIF-4E undergo turnover through the general 5′→3′ pathway. mRNA half-lives were measured in strains containing mutations in both eIF-4E and DCP1 and in strains containing mutations in both eIF-4E and XRN1. Steady-state half-life measurements of the PGK1pG mRNA were determined from agarose Northern gels. The numbers above the lanes indicate minutes after transcriptional repression. The average value (minutes) of the half-life for the full-length transcript from at least three experiments in each strain is given on the right. Northern gels were probed with oligonucleotide oRP141. ts, temperature sensitive.