TABLE 2.
Effects of translation initiation factors on mRNA half-life and deadenylation of PGK1 and MFA2
| Strain | PGK1 RNA half-lifea (min) | PGK1 deadenylation rateb (A’s/min) | MFA2 RNA half-lifea (min) |
|---|---|---|---|
| Wild type | 17.0 | 3.1 | 4.0 |
| caf20Δ | 20.0 | NDc | 4.4 |
| eIF-4G2Δ | 11.6 | ND | 2.6 |
| eIF-4G1Δ | 8.0 | 3.7 | 2.0 |
| ts eIF-4E cdc33-42 | 6.0 | 4.1 | 3.0 |
| ts eIF-4A | 8.5 | 4.5 | 3.5 |
| gcd10-505 | 4.9 | ND | 2.3 |
| prt1-63 | 6.0 | 4.0 | 3.0 |
| ts eIF-4E prt1-63 | 4.0 | 5.1 | 2.0 |
a
Comparative half-life measurements for PGK1 and MFA2 mRNA based on a minimum of three transcription shutoff experiments. The experimental variation for the half-life measurements of each strain was less than ±15%.
b
Deadenylation rates of PGK1 are given for all the translation initiation mutants based on measurements taken from transcriptional shutoff mRNAs run on acrylamide Northern gels. The deadenylation rates are based on a minimum of two transcription shutoff experiments. The experimental variation for the half-life measurements of each strain was less than ±15%.
c
ND, not determined.