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. 1999 Aug;19(8):5257–5266. doi: 10.1128/mcb.19.8.5257

FIG. 4.

FIG. 4

Growth defects of strains containing the tef5 mutant alleles. (A) Strains containing the lys2-801 allele (UAG) and either the wild-type TEF5 gene or one of the tef5-1 to tef5-10 alleles on a LEU2 CEN plasmid were grown at 30°C, and equal numbers of cells were spotted on YEPD medium. From top left are shown TKY235 (TEF5), TKY238 (tef5-1), TKY240 (tef5-2), TKY244 (tef5-3), TKY236 (tef5-4), TKY242 (tef5-5), TKY251 (tef5-6), TKY243 (tef5-7), TKY239 (tef5-8), TKY241 (tef5-9), and TKY237 (tef5-10). Growth was monitored following 3 to 7 days at 37, 30, 24, or 13°C. (B) Growth was monitored on complete medium–0.5 mg of paromomycin per ml (top) and C-Lys–0.5 mg of paromomycin per ml (bottom) for strains (from top left) TKY235 (TEF5), TKY238 (tef5-1), TKY240 (tef5-2), TKY244 (tef5-3), and TKY243 (tef5-7) following 7 days at 30°C.