TABLE 5.
Reading of nonsense codons in strains containing tef5 alleles
| Allele | Mutation | % Misreadinga
|
||
|---|---|---|---|---|
| UAA | UAG | UGA | ||
| TEF5 | None | 0.88 ± 0.29 | 1.3 ± 0.36 | 1.2 ± 0.66 |
| tef5-1 | S121L | 0.28 ± 0.12 | 0.55 ± 0.08 | 0.33 ± 0.17 |
| tef5-2 | S121I | 0.32 ± 0.22 | 0.31 ± 0.06 | 0.36 ± 0.16 |
| tef5-3 | S121N | 0.29 ± 0.11 | 0.24 ± 0.06 | 0.48 ± 0.30 |
| tef5-7 | K120R S121Δ I122Δ | 0.21 ± 0.08 | 0.18 ± 0.07 | 0.35 ± 0.17 |
a
Misreading was monitored by determining the β-galactosidase activity in cells containing either a wild-type lacZ reporter gene or a variant containing the indicated stop codon in frame with lacZ. Percent misreading is the β-galactosidase activity for the indicated nonsense reporter divided by the activity for the wild-type lacZ reporter.