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. 2021 Aug 24;13(16):20598–20608. doi: 10.18632/aging.203450

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Copyright: © 2021 Xie et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Hsa_circ_0091994 knockdown promoted the apoptosis and suppressed the wound healing activity in AGS and HGC-27 cells. AGS and HGC-27 cells were treated with shRNA-1 or shRNA-ctrl for 48 hr. (A, B) Cell apoptosis rate was measured in each group. (C, D) Wound healing assay was employed to evaluate the effect of hsa_circ_0091994 knockdown on wound healing activity of AGS and HGC-27 cell. (E) The protein expression of E-cadherin and α-SMA was examined using western blot assay. β-actin was used as a loading control. (F, G) The expressions of E-cadherin and α-SMA were quantified respectively. The**P<0.01, compared with blank; n = 3.