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. 2021 Aug 25;13(16):20534–20551. doi: 10.18632/aging.203435

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Copyright: © 2021 Liao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Inhibition of NLRP3 by MCC950 attenuated cardiocytes aging induced by D-gal in H9c2 cells. H9c2 cells were pre-treated with or without MCC950 (10μM), a commonly used NLRP3 inhibitor, for 1 hour, and then incubated with or without 10g/L D-gal for 24 hours. (A) Representative bright-field photomicrographs showed that MCC950 treatment decreased the percentage of cells expressing β-galactosidase. (B) Flow cytometry analysis was applied to detect the β-galactosidase mean fluorescence intensity after the MCC950 treatment. (C) The CellEvent™ Senescence Green staining showed that MCC950 treatment decreased the senescence-associated β-galactosidase expression induced by D-gal. (D) The aging-associated proteins (P53, P21) were detected by western blot, and the corresponding quantification was present.