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. 2021 Aug 25;13(16):20534–20551. doi: 10.18632/aging.203435

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Copyright: © 2021 Liao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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ROS generation was similar when NLRP3 inflammasomes were inhibited in the cardiocytes aging model. H9c2 cells were pre-treated with or without MCC950 (10μM), a commonly used NLRP3 inhibitor, for 1 hour, and then incubated with or without 10g/L D-gal for 24 hours. (A) ROS generation was detected using a DCFH-DA probe. Representative fluorescent images showed that ROS generation was similar no matter NLRP3 inflammasomes were inhibited or not in the cardiocytes aging model. (B) Intracellular ROS was quantified by flow cytometry.