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. 2021 Aug 19;13(16):20383–20394. doi: 10.18632/aging.203420

Figure 3.

Figure 3

MiR-224-5p is targeted and negatively regulated by hsa_circ_0134111. (A) Diagram showing binding sites for miR-224-5p in the hsa_circ_0134111 sequence and nucleotide sequence of the mutant hsa_circ_0134111 construct. (B) Chondrocytes were treated with miR-224-5p NC, miR-224-5p agomir, or miR-224-5p antagomir and RT-qPCR was performed to measure miR-224-5p levels in cells. (C) A dual-luciferase reporter assay was used to evaluate binding of miR-224-5p to hsa_circ_0134111. (D) FISH experiment was performed to detect the co-localization of hsa_circ_0134111 and miR-224-5p in human primary chondrocytes. (E) CCK-8 assay was used to detect cell viability. (F, G) Flow cytometric analysis of apoptosis. (H) Chondrocytes were treated with miR-515-5p NC or miR-515-5p antagomir and RT-qPCR was performed to measure miR-515-5p levels in cells. (I) CCK-8 assay was performed to detect cell viability. **P<0.01, compared with control; ##P<0.01, compared with IL-1β. ^^P<0.01, compared with IL-1β + hsa_circ_0134111 siRNA1.