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. 2021 Aug 23;59(1):1148–1158. doi: 10.1080/13880209.2021.1961820

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© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — YGJ inhibits the activation of hepatic stellate cells and fibrosis. (a) Sirius red staining (×100). (b) α-SMA immunostaining (×200). (c) Col (1) immunostaining (×200). (d) Sirius Red-positive region. (e) α-SMA and Col(1) protein bands were depicted in immunoblots, and (f) the histogram is a densitometric analysis of the protein bands (n = 5 per group). (g) α-SMA, Col(1) and TGF-β mRNA expressions were measured by RT-PCR and normalized to GAPDH mRNA (n = 5 per group). **p < 0.01. N: untreated group (control); 2-AAF/CCl₄: 2-acetylaminofluorene/carbon tetrachloride-treated group; YGJ: 2-AAF/CCl₄ + Yiguanjian decoction-treated group; SORA: 2-AAF/CCl₄ + sorafenib-treated group.