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. 2021 Aug 23;59(1):1148–1158. doi: 10.1080/13880209.2021.1961820

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© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — YGJ inhibits liver inflammation and bile duct reaction. (a) H&E staining (×200). (b) CD68 immunostaining (×200). (c) CD163 immunostaining (×200). (d) CK19 immunostaining (×200). (e) CK19 protein bands were depicted in the immunoblot images, and (f) the densitometric quantification of the protein bands presented as a histogram (n = 5 per group). (g) CD68, CD163, and TNF-α mRNA expressions were measured by RT-PCR and normalized to GAPDH mRNA (n = 5 per group). (h) Serum levels of alanine aminotransferase and aspartate aminotransferase. *p < 0.05 and **p < 0.01. N: untreated group (control); 2-AAF/CCl₄: 2-acetylaminofluorene/carbon tetrachloride-treated group; YGJ: 2-AAF/CCl₄ + Yiguanjian decoction-treated group; SORA: 2-AAF/CCl₄ + sorafenib-treated group.