Table 1.
Primer pairs and probes used for Real-time PCR.
| Gene | Primer sequence | Gene | Primer sequence | ||
|---|---|---|---|---|---|
| α-SMA | Forward | AATGGCTCTGGGCTCTGTAA | Wnt 1 | Forward | GGGGAGCAACCAAAGTCG |
| Reverse | TCTCTTGCTCTGGGCTTCAT | Reverse | TGGAGGAGGCTATGTTCACG | ||
| Col1 | Forward | TGACTGGAAGAGCGGAGAGT | Wnt 3 A | Forward | TCCGACTCTTGGCAGAACTT |
| Reverse | GACGGCTGAGTAGGGAACAC | Reverse | AATGGAATAGGTCCCGAACA | ||
| TGF-β1 | Forward | ATTCCTGGCGTTACCTTGG | Wnt 4 | Forward | GGCACTCATGAACCTTCACAACA |
| Reverse | AGCCCTGTATTCCGTCTCCT | Reverse | CTTTACCTCACAGGAGCCTGACAC | ||
| TNF-α | Forward | GACGTGGAACTGGCAGAAGAG | Wnt 5 A | Forward | GCGCTGCTGGAGTGGTAAAT |
| Reverse | TTGGTGGTTTGTGAGTGTGAG | Reverse | AGCCAGTCCCGAGGTAAGTC | ||
| CD68 | Forward | GGACCCACAACTGTCACTCAT | Wnt 5B | Forward | CGAGCCCTCATGAACTTACAGAAC |
| Reverse | AAGCCCCACTTTAGCTTTACC | Reverse | GGAGACTCCGTGACATTTGCAG | ||
| CD163 | Forward | TGGGATCGCCGTGACGCTTC | Wnt 8 A | Forward | CCTGGGAGCGGTGGAACT |
| Reverse | CAGCGACTGCCTCCACCGAC | Reverse | CCTGGTGTGGGTTGAAAACTG | ||
| OV6 | Forward | GATGCTGGACACAAACTCAACT | Wnt 8B | Forward | AAGGCTTACCTGGTCTACTC |
| Reverse | GCC ACA ACAGGA ATCTCTCC | Reverse | CAGAGCTGATGGCGTGCACA | ||
| SOX9 | Forward | GAA AGA CCA CCCCGATTACAAG | Wnt 10B | Forward | CCTCAAGCGCGGTTTCC |
| Reverse | AAGATGGCGTTAGGA GAGATGTG | Reverse | CAGCAGCCAGCATGGAGAA | ||
| EpCAM | Forward | TGTGGACATAGCTGATGTGGCTTA C | Wnt 11 | Forward | TTGACCTGGAGAGAGGTACAC |
| Reverse | CACCCTCAGGTCCATGCTCTT A | Reverse | GTCAGGGGAGCTCTGTAGATA | ||
| CK19 | Forward | TATCTGGATCTGCGTAGTGTGG | Frizzled 1 | Forward | GGGAATGCAGTCACCAGTACCA |
| Reverse | ATACAA AACCAA ACTGGGGATG | Reverse | CCAGACCCATAGCAGGTTCCA | ||
| Hep | Forward | TAGCAGAGATGAGCCGTGTG | Frizzled 2 | Forwa rd | ACTGCAAGAGCCTAGCCAT CC |
| Reverse | GCTTTGAGGCAGGCGTATT | Reverse | ATCCAGAAGCCCGACGTGA | ||
| STAT1 | Forward | GTCCTCTTCCAGCAGCTCATTCAG | Frizzled 3 | Forward | ACACATGGCACCAGCATGAAC |
| Reverse | ACCAACAGTCTCAGCTTCACAGTG | Reverse | CCATGCGAAGGCCAAGACTAA | ||
| IRF3 | Forward | GGCTTACGACAGGACGCACAG | Frizzled 4 | Forward | GACAACTTTCACGCCGCTCA |
| Reverse | CAGGTTGACAGGTCTGGCTTATCC | Reverse | TTCAGGACTGGTTCACATCGTCTC | ||
| IRF5 | Forward | GCTCCATCACATCTGGCAGTCC | Frizzled 5 | Forward | CGAGAGCACAGCCACATTCACTA |
| Reverse | GGAAGTCCAAGTCAGCCACCATC | Reverse | GAGCTGGCCATGCCAAAGA | ||
| IRF8 | Forward | TGGACATTTCCGAGCCATACAA | Frizzled 6 | Forward | CAGCAGCGTCCAACTCCAAG |
| Reverse | CGATCTCTGAACGGCCACAC | Reverse | TGCACTCCATCAGGCCAGTC | ||
| NF-κB | Forward | GATGGGACGACACCTCTACACATA | LRP 5 | Forward | GACATTTACTGGCCCAATGG |
| Reverse | CCCAAGAGTCGTCCAGGTCA | Reverse | CTGCCCTCCACCACCTTCT | ||
| SOSC3 | Forward | TCAACGGTCACCTGGACTCCTA | LRP 6 | Forward | TCTCCGGCGAATTGAAAG |
| Reverse | GGTCCAGGAACTCCCGAATG | Reverse | GAGTCTTCTAGCACGATCCTGT | ||
| β-catenin | Forward | GTCTGAGGACAAGCCACAGGACTAC | GAPDH | Forward | AAGGTCATCCATGACAACTTTGGC |
| Reverse | AATGTCCAGTCCGAGATCAGCA | Reverse | ACAGTCTTCTGGGTGGCAGTGAT |