Figure 3.

Spd modulates FGF21 protein expression in liver and white adipose tissues. FGF21 protein levels were determined by Western blots from liver, sub. fat, and vis. fat of (A) young mice, (B) old mice, and (C) young mice under HFD after 6 mo of Spd treatment. Relative FGF21 levels (normalized to GAPDH) were quantified underneath. All bars represent mean ± SEM. Significant differences were taken when p <.05. FGF21 = fibroblast growth factor 21; GAPDH = glyceraldehyde 3-phosphate dehydrogenase; HFD = high-fat diet; PBS = phosphate-buffered saline; Spd = spermidine; sub. fat = subcutaneous fat; vis. fat = visceral fat.