Figure 6.

IL-1β expression by alveolar leukocytes from patients with direct acute respiratory distress syndrome (ARDS) is inhibited by tetracycline (TET). (A) BAL fluid from patients with direct ARDS was collected as described in Figure 1 and analyzed for neutrophils and macrophages via flow cytometry. Leukocytes were cultured and coincubated with increasing doses of (B and C) TET and (D) COL-3 (1, 3, 10, and 30 μg/ml) for 16 hours, and indicated cytokine production was quantified by ELISA. *P < 0.05 (vs. TET 0 μg/ml). Median with interquartile range of six patients; Mann-Whitney test.