Table 2 |.
Summary of CRC diagnostic statistics for conventional and EV markers.
| Markers | Cutoff | Training cohort (n = 83) | Testing cohort (n = 48) | ||||||
|---|---|---|---|---|---|---|---|---|---|
| AUC | Sensitivity (%) | Specificity (%) | Accuracy (%) | Sensitivity (%) | Specificity (%) | Accuracy (%) | |||
| Serum | CEA | 1.65 | 0.85 | 79 | 88 | 82 | 76 (58–89) |
60 (32–84) |
71 (56–83) |
| CA199 | 12.9 | 0.74 | 64 | 84 | 70 | 52 (34–69) |
87 (61–98) |
63 (48–77) |
|
| EV single markers | EGFR | 0.23 | 0.67 | 59 | 84 | 66 | 52 (34–69) |
87 (60–98) |
63 (48–77) |
| EpCAM | 0.08 | 0.91 | 86 | 100 | 90 | 85 (68–95) |
93 (68–100) |
88 (75–95) |
|
| CD24 | 0.60 | 0.77 | 55 | 100 | 69 | 39 (23–58) |
100 (78–100) |
58 (43–72) |
|
| GPA33 | 0.02 | 0.65 | 47 | 96 | 61 | 42 (25–61) |
73 (45–92) |
52 (37–67) |
|
| CD133 | 0.03 | 0.70 | 66 | 76 | 69 | 58 (39–75) |
53 (27–79) |
56 (41–71) |
|
| EGFR + EpCAM | 2.69 | 0.94 | 90 | 100 | 93 | 88 (72–97) |
100 (78–100) |
92 (80–98) |
|
| EGFR + CD24 | 3.15 | 0.86 | 60 | 90 | 71 | 45 (28–64) |
100 (78–100) |
63 (47–76) |
|
| EpCAM + CD24 | 4.23 | 0.97 | 91 | 100 | 94 | 88 (72–97) |
100 (78–100) |
92 (80–98) |
|
| EV weighted combination | EGFR + EpCAM + CD24 | 4.23 | 0.97 | 91 | 100 | 94 | 88 (72–97) |
100 (78–100) |
92 (80–98) |
| EGFR + EpCAM + CD24 + CD133 | 6.15 | 0.99 | 95 | 100 | 96 | 88 (72–97) |
93 (68–100) |
90 (77–97) |
|
| EGFR + EpCAM + CD24 + GPA33 | 4.96 | 0.98 | 97 | 100 | 98 | 94 (80–99) |
100 (78–100) |
96 (85–99) |
|
| EGFR + EpCAM + CD24 + GPA33 + CD133 | 5.63 | 0.99 | 98 | 100 | 99 | 100 (89–100) |
93 (68–100) |
96 (89–100) |
|