Fig 2. Coronary vasoconstricting responses to serotonin before and after hydroxyfasudil.

Representative CAG at 4 weeks (A, D) after intracoronary nitroglycerin (10 μg/kg), (B, E) serotonin (100 μg/kg), and (C, F) serotonin after pre-treatment with hydroxyfasudil (a selective Rho-kinase inhibitor, 300 μg/kg). Coronary hyperconstricting responses at the stent edges (blue and red arrows) of EES (yellow lines) were markedly suppressed in the LIPUS group compared with the sham group (B, E), which were fully abolished by hydroxyfasudil (C, F). (G) Coronary vasoconstriction to serotonin was significantly attenuated in the LIPUS group compared with the sham group, which was prevented by hydroxyfasudil. (H) Vasoconstriction was not evident in the control arteries. Results are expressed as mean±SEM. SEM = standard error of mean; other abbreviations as in Fig 1.