FIG. 2.

(A) Western blot analysis of the translocation IN-CPY in wild-type or cer1Δ cells. In pep4 strains, the lower band is ppCPY, while in a PEP4 strain, it is mature CPY. (B) CPY can be posttranslationally translocated in vivo. Pulse-chase analysis of CPY folding done as described in the legend to Fig. 1 was carried out. (Top) The kar2-191 strain was kept at 25°C for the entire experiment. (Bottom) The labeling was done at 37°C, and the chase was done at 25°C. The lower band represents ppCPY and mature CPY because the kar2-191 strain is PEP4.