Figure 3. Intra-host variant detection on time series dataset.

a) Venn diagram showing counts of variant calls shared between Lofreq on illumina sequencing runs and Variabel and Clair3 on nanopore sequencing runs on the same samples from time series dataset b) False positive rates at different variant allele frequencies and cumulative count of false positive variant calls of Variabel and Clair3 for time series dataset, c) Precision, recall, and f-score comparison of Lofreq default, Clair3, and Variabel on both time series dataset and cross patient dataset. Significance between Clair3 and Variabel were calculated using the two-sided paired t-test. Significance labeling: n.s.(P>0.05), *(P≤0.05), **(P≤0.01), ***(P≤0.001).