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. 2021 Aug 30;11:733595. doi: 10.3389/fonc.2021.733595

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Copyright © 2021 Xia, Zhang, Xia, Zhu, Liu, Xu and Yuan

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effect of WAC-AS1 on glycolysis and the cell cycle. (A) cell cycle analysis was used to detect the cell cycle distribution after transfection; (B) glucose uptake in Hep3B and HCCLM3 cells; (C) lactate production detected by the lactate assay kit; (D) CCK-8 assay for cell viability; (E) cell viability of Huh7 cells after overexpression of WAC-AS1; (F) representative images of plate colony formation in Huh7 cells; (G) cell cycle analysis of Huh7 cells; (H) glucose uptake and lactate production in Huh7 cells; (I) ECAR levels in Hep3B cells; (J) subcutaneous xenograft tumors and growth curve. ∗P < 0.05; ∗∗P < 0.01; ***p < 0.001.