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. 2021 Aug 30;11:733595. doi: 10.3389/fonc.2021.733595

Figure 8.

Figure 8

WAC-AS1 regulated ARPP19 by sponging miR-320d. (A) venn diagram showing the predicted target genes of WAC-AS1 from databases (miRDB, ENCORI, and starBase); (B) screening out target genes with differences analysis; (C) venn diagram showing the predicted target genes of miR-320d; (D) the correlation analysis between WAC-AS1 and ARPP19 using data from TCGA database; (E) the wild-type and the mutated sequences of the ARPP19 mRNA 3’-UTR (mutation site: red); (F) the levels of miR-320d after miR-320d mimics and miR-320d inhibitor were transfected; (G) expression of the ARPP19 after treated with miR-320d mimics or inhibitor; (H) the luciferase activity in luciferase reporter plasmid containing wild-type ARPP19 and mutated ARPP19 co-transfected with miR-320d mimics or negative control; (I) the wild-type and the mutated sequences of the WAC-AS1; (J) the luciferase activity in luciferase reporter plasmid containing wild-type WAC-AS1 and mutated WAC-AS1 co-transfected with miR-320d mimics or negative control; (K) the protein levels of ARPP19 were regulated by WAC-AS1 and miR-320d; (L) ARPP19 expression in differentially treated cells; (M) glucose uptake in Hep3B and HCCLM3 cells; (N) lactate production detected by the lactate assay kit. ∗∗P < 0.01; ***p < 0.001.