FIG 5.

Serum ApoA1 concentrations are decreased in DENV-infected patients. (A) Total ApoA1 was quantified in human serum using the human ApoA1 ELISA kit (Thermo Fisher Scientific). Of samples from 33 patients, 23 were DENV positive and 10 were DENV negative. Samples from 7 healthy donors were also quantified as control samples. (B) Among DENV-positive patients, 16 were diagnosed with dengue fever and 7 were diagnosed with severe dengue. Bars represent mean and SDs. (C) HepG2 cells were mock infected or infected with DENV2 (multiplicity of infection of 1) for 48 h, and total cell lysates were analyzed by Western blotting. The ApoA1 band was quantified and expressed relative to glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Bars represent means and SDs from three experiments. Asterisks represent significant differences, compared to mock-infected cells. ****, P < 0.005; ns, not significant.