FIG 2.

Molecular evolution of feline A3Z3 genes and the position of the residues under diversifying selection. (A and B) Codon sites under diversifying selection in feline A3Z3 genes. The P value for each codon site is plotted. The site with indel mutation was omitted from the result. The results by FEL (37) (A) and MEME (38) (B) are shown. FEL and MEME are specialized for detecting the selections that occurred in the entire phylogenetic tree and the specific clades, respectively. (C and D) Structure modeling of domestic cat A3Z3 (hap I). The data of the structure homology model are from our previous study (22). Cartoon (C) and surface (D) models of the structure of domestic cat A3Z3 hap I are shown. In panels C and D, the residue under diversifying selection estimated by both FEL and MEME (residue 65) and those by FEL (residues 32, 49, 74, and 127) are represented in red and orange, respectively. Residue 66 is labeled in blue. Note that the residue 186 is not plotted, because this residue is located outside this homology model.