TABLE 3.

Property comparison of PersiAmy1 with some known cold-active and/or alkaline α-amylases.

Source	pH stability	Thermal stability	Cold activity	References
Bacillus SP-CH7	>80% activity after 60 min at pH 13.0	<20% activity after 60 min at 95°C	<20% activity at 15°C	Priyadarshini and Ray, 2019
Bacillus subtilis N8	50% activity after 24 h at pH 12.0	83% activity after 60 min at 40°C	91% activity at 10°C	Arabacı and Arıkan, 2018
Pseudoalteromonas M175	<80% activity after 60 min at pH 9.0	<10% activity after 60 min at 50°C	<50% activity at 0°C	Wang et al., 2018
Bacillus mojavensis	<70% activity after 60 min at pH 9.0	<40% activity after 60 min at 70°C	40% activity at 30°C	Hammami et al., 2018
Geomyces pannorum	80% activity after 30 min at pH 9.0	0% activity after 15 min at 60°C	<30% activity at 0°C	He et al., 2017
Exiguobacterium SH3	–	<10% activity after 30 min at 60°C	41% activity at 0°C	Emampour et al., 2015
Arthrobacter agilis	<60% activity after 60 min at pH 9.0	>80% activity after 60 min at 70°C	–	Kim et al., 2017
Bacillus methylotrophicus	<100% activity after 60 min at pH 9.0	<60% activity after 60 min at 50°C	<20% activity at 20°C	Hmidet et al., 2019
Bacillus pseudofirmus	80% activity after 12 h at pH 9.0	<80% activity after 20 min at 50°C	40% activity at 30°C	Lu et al., 2016
Metagenome-derived	<80% activity after 30 min at pH 9.0	<10% activity after 30 min at 90°C	<20% activity at 2°C	Nair et al., 2017
Metagenome-derived	>80% activity after 14 h at pH 9.0	<10% activity after 60 min at 50°C	70% activity at 1°C	Vester et al., 2015
Metagenome-derived (PersiAmy1)	86% activity after 180 min at pH 9.0	61% activity after 180 min at 90°C	87% activity at 0°C	This study