Table 1.
Strains and plasmids used in this study
| Strain/plasmid | Relevant genotype/phenotype | Antibiotic (C, μg/ml) for strains selection | Reference /source |
|---|---|---|---|
| E. coli | |||
| DH5α | Sangon | ||
| BL21 | Sangon | ||
| S. pneumoniae | |||
| JH1900 |
S. pneumoniae D39 strain, Capsulated strain serotype 2, rpsl K56T |
Smr: 150 | lab stock [22] |
| JH0001 | D39ΔdexB-cps2A::JC1 | Kanr: 200 | present study |
| JH0002 | D39ΔdexB-cps2A | Smr: 150 | present study |
| JH1101 | D39ΔmgaSpn | ermr: 100 | present study |
| JH1102 | D39ΔdexB-cps2AΔmgaSpn | ermr: 100 | present study |
| JH1104 | D39ΔmgaSpn:: mgaSpn | specr: 50 | present study |
| JH1106 | D39ΔdexB-cps2AΔmgaSpn:: mgaSpn | specr: 50 | present study |
| TH7898 | D39s, ΔbgaA::Pef-tu-kan-rpsl | Kanr: 200 | lab stock [22] |
| Plasmids | |||
| pPEPZ-Plac | Cloning vector | specr: 50 | Addgene [56] |
| pPEPZ-mgaSpn | Contains mgaSpn | specr: 50 | present study |
| pET28-mgaSpn | Cloning vector | Kanr: 50 | present study |