Figure 8.

The relationship between ABCC5 and immune cells. (a)Compared with B-cell and T_cell, the ABCC5 gene was mainly expressed in Macrophage cells (P < 0.001). (b)The differentiation trajectory of immune cells. The darker the blue, the earlier the differentiation occurred, that is, the cells differentiated from the dark blue branches to the lighter blue branches. We found that the upper right corner of (Figure 8(b)) indicates seven differentiated states of immune cells, with the red at the bottom being the earlier ones. The figure in the lower-left corner of (Figure 8(b)) indicates that with the differentiation of immune cells, the earliest differentiated cells may be T-cell, followed by B cell and macrophage. The lower-right corner of (Figure 8(b)) shows the distribution of different immune cell clusters during the differentiation of immune cells, that is, red Cluster 5 at the bottom may start to differentiate first, then differentiate into Cluster 7 and 8, and finally differentiate into Cluster 6. (c)During the differentiation of immune cells, the expression changes of gene ABCC5 and its co-expressed genes were grouped into five modules. For example, in the first module, the gene expression was mainly increased first and then decreased (including PDCL, DNAJC18, METTL8, etc.). ABCC5 gene was included in the pink module, suggesting that with the differentiation of immune cells, the expression of ABCC5 and other genes in the module first increased, then decreased, and then increased, which may indicate that ABCC5 plays an important role in the differentiation of immune cells. (d)Expression of transcription factors related to the ABCC5 gene during the differentiation of immune cells, and the results suggested that the expression of transcription factors related to the ABCC5 gene also changed during the differentiation of immune cells. (e)The ABCC5 gene was singled out to explore its specific expression changes during the differentiation of immune cells. It was found that with the development of differentiation, the expression of the ABCC5 gene first decreased, and then increased again when it reached a certain value, suggesting that ABCC5 may play a certain role in the differentiation of immune cells in HCC. (f)GO analysis found that the coexpressed genes of ABCC5 were mainly related to RNA synthesis and splicing modification