Figure 2.

AK-nanoparticles targeted TC-1 cells and expressed exogenous protein

FITC-AK and FITC-GK nanoparticles were incubated with TC-1 cells or HAVSMC cells (a), or 2 μg/μl of AK-labeled Rhodamine and a series of the indicated concentration of free RGD-GGG cyclic peptides were co-incubated with TC-1 cells (b), and the fluorescence on cells was determined by fluorescence microscope. (c) TC-1 cells were incubated with nanoparticles AK/E7, AK/HSP110, AK/E7-HSP110 or PBS. The cells were then subjected to 10% SDS-PAGE and subsequent Western-blotting assays with an anti-HSP110 antibody respectively. GAPDH was included as an internal control. *P< .01 vs other controls.