FIG. 3.
PER and TIM do not inhibit the DNA binding activity of the non-bHLH-PAS transcription factor c-Rel. Reticulocyte lysates containing 7 fmol of c-Rel (lanes 3 to 5), 14 fmol of IκBα (lane 4 and 6), or 14 fmol each of PER and TIM (lane 5) were incubated with a 32P-radiolabeled synthetic oligonucleotide containing NF-κB DNA binding sites (lanes 1 to 6), and protein-DNA complexes visualized as described in the legend to Fig. 1. The positions of a complex formed by a c-Rel homodimer (top arrowhead) and an endogenously produced complex (bottom arrowhead) that is also observed when using nonprogrammed reticulocyte lysate (lane 2) are indicated.